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Pre-Meeting Workshops - Annual Meeting and Exhibition 2006
Sunday, July 30
 All
workshops will be held at the Center of Marine Biotechnology,
University of Maryland Biotechnology Institute.
#1: Cell Culture
Techniques (wet workshop)
Conveners: Winona Wagner, DuPont
and Julia Cino, New Brunswick Scientific, Co.
This workshop
will provide hands-on experience with bench top bioreactors.
Participants will prepare an autoclavable bioreactor vessel
as configured for a suspension culture run. Vessel configurations
for the culture of anchorage dependent cells will be demonstrated.
Participants will learn how to calibrate DO and pH probes,
observe a running culture and review data captured from the
process. Laboratory work will be augmented by presentations
and discussions on media development, equipment (sensors,
controllers) & methods (control strategies), scale-up and
downstream processing. Faculty
#2: Protein Expression: Things to Consider When Expressing
a New Protein
Convener: Robert Petrovich, NIH/NIEHS
In this post-genomics era, one of the biggest hurdles is
how to express the protein of interests. In this workshop
we will discuss some approaches to the expression of a new
target protein. The discussion will cover our experiences
with: different affinity/solubility tags, how media composition
and growth conditions can effect solubility/expression levels,
what to consider when choosing a protease to remove the tag
and some of the problems encountered in scale up and how
to approach overcoming these problems. We will also discuss
some aspects of protein refolding. The participants should
gain a basic understanding some of the tools available when
considering the expression of a new protein. Faculty; Schedule
#3: Design and Optimization of Growth Medium
Conveners: Randy Greasham, Consultant
Medium design and optimization are key components in the
development of a highly productive, consistent and scaleable
fermentation process. Rapid process development requires
these components to be developed in a time and resource efficient
manner. This workshop shall focus on the physiological logic
behind defining the nutritional components of growth medium,
the control of the growth and production phases through process
engineering, and their efficient optimization. A primary
focus of the course will be a practical overview of the use
of Statistical Design of Experiment in the optimization of
growth medium and process conditions. The content of this
workshop shall apply to all fermentations from bacteria to
cell culture. Those attending should gain sufficient practical
knowledge, literature, and supporting materials to implement
a media design and optimization program. Faculty
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